A NEW DIAZO REAGENT FOR SPECIFIC STAINING OF CONJUGATED BILIRUBIN IN TISSUE SECTIONS

A new diazo method for the determination of bilirubin.

A new technic for the determination of total bilirubin is reported. The substitution of 2,4 dichloroaniline for sulfanilic acid results in a very stable diazotized reagent which reacts quickly with bilirubin in plasma or serum. Comparison with a sulfanilic acid procedure indicates that similar analytic values are obtained with this technic; short quality control studies indicate satisfactory pr...

A Device for Staining Tissue Sections for Electron Microscopy

It has been the experience of this laboratory that the lead hydroxide stain of Watson (1) gives excellent results in increasing contrast in thin sections of tissue, but that the stain is inconvenient to use because on contact with air it forms an insoluble precipitate which contaminates both the stored solution and the sections. 1 Contamination introduced in transferring the solution from the s...

Non-nuclear staining of thick tissue sections.

Microfluidic Probe for Advanced Staining of Human Tissue Sections

A key challenge in diagnostic pathology is to provide accurate and precise diagnostics with limited excised tissue. It is also increasingly important to test the tissue samples against multiple markers to enhance the confidence in results and sub-type the diseases for personalized treatment therapies. To this end, we performed local chemical reactions and staining on tissue sections using a sca...

Staining of Tissue Sections for Electron Microscopy with Heavy Metals

Descriptions of three heavy metal stains and methods of application to tissue sections for electron microscopy are presented. Lead hydroxide stains rather selectively two types of particles in liver: those associated with the endoplasmic reticulum and containing ribonucleic acid and other somewhat larger particles. Barium hydroxide emphasizes certain bodies within vesicles of the Golgi region o...